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pc-plc inhibitor d609  (Biomol GmbH)

 
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    Biomol GmbH pc-plc inhibitor d609
    Pc Plc Inhibitor D609, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc-plc+inhibitor+d609/d609/pm18403015-48-3-26
    Average 90 stars, based on 1 article reviews
    pc-plc inhibitor d609 - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Involvement of phosphatidylinositol-phospholipase C in immune response to Salmonella lipopolysacharide in chicken macrophage cells (HD11).
    Article Snippet: The activation of phospholipases is one of the earliest key events in receptor-mediated cellular responses to a number of extracellular signaling molecules.. Lipopolysaccharide (LPS) is a principle component of the outer membrane of Gram-negative bacteria and a prime target for recognition by the innate immune system.. In the present study, we evaluated the role of specific phospholipase in the activation of a chicken macrophage cell line HD11 by LPS.

    Article Title: Phospholipase C, phosphatidylinositol 3-kinase, and intracellular [Ca(2+)] mediate the activation of chicken HD11 macrophage cells by CpG oligodeoxynucleotide.
    Article Snippet: e@ffsru.usda.gov Summary The activation of phospholipases is one of the earliest key events in receptor-mediated cellular responses to a number of extracellular signaling molecules.. Oligodeoxynucleotides containing CpG motifs (CpG ODN) mimic microbial DNA and are immunostimulatory to most vertebrate species.. In the present study, we used the production of nitric oxide (NO) as an indicator to evaluate the involvement of the signaling cascades of phospholipases and phosphatidylinositol 3-kinase (PI3K) in the activation of chicken HD11 macrophage cells by CpG ODN.



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    Fas stimulation in NPD lymphoblasts efficiently induces <t>PC-PLC</t> activity, but fails to generate GD3 ganglioside accumulation. Cell extracts from 10 7 normal ( A ) and NPD ( B ) lymphoblasts treated for 15 min with control IgM or 500 ng/ml anti-Fas mAb, preceded or not by preincubation with 50 μg/ml <t>D609</t> for 30 min, were reacted with radiolabeled PC, and the released PCho was quantitated. ( C ) 10 7 normal and NPD lymphoblasts were left untreated or were stimulated with 100 μM C 6 -ceramide ( cer ) or 500 ng/ml anti-Fas for 5 or 15 min, respectively. Then polar lipids were extracted and run on HPTLC plates along with GD3 standard ( st ), and GD3 was detected by anti-GD3 immunostaining. Four experiments gave comparable results.
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    Fas stimulation in NPD lymphoblasts efficiently induces PC-PLC activity, but fails to generate GD3 ganglioside accumulation. Cell extracts from 10 7 normal ( A ) and NPD ( B ) lymphoblasts treated for 15 min with control IgM or 500 ng/ml anti-Fas mAb, preceded or not by preincubation with 50 μg/ml D609 for 30 min, were reacted with radiolabeled PC, and the released PCho was quantitated. ( C ) 10 7 normal and NPD lymphoblasts were left untreated or were stimulated with 100 μM C 6 -ceramide ( cer ) or 500 ng/ml anti-Fas for 5 or 15 min, respectively. Then polar lipids were extracted and run on HPTLC plates along with GD3 standard ( st ), and GD3 was detected by anti-GD3 immunostaining. Four experiments gave comparable results.

    Journal: The Journal of Experimental Medicine

    Article Title: Acidic Sphingomyelinase (ASM) Is Necessary for Fas-induced GD3 Ganglioside Accumulation and Efficient Apoptosis of Lymphoid Cells

    doi:

    Figure Lengend Snippet: Fas stimulation in NPD lymphoblasts efficiently induces PC-PLC activity, but fails to generate GD3 ganglioside accumulation. Cell extracts from 10 7 normal ( A ) and NPD ( B ) lymphoblasts treated for 15 min with control IgM or 500 ng/ml anti-Fas mAb, preceded or not by preincubation with 50 μg/ml D609 for 30 min, were reacted with radiolabeled PC, and the released PCho was quantitated. ( C ) 10 7 normal and NPD lymphoblasts were left untreated or were stimulated with 100 μM C 6 -ceramide ( cer ) or 500 ng/ml anti-Fas for 5 or 15 min, respectively. Then polar lipids were extracted and run on HPTLC plates along with GD3 standard ( st ), and GD3 was detected by anti-GD3 immunostaining. Four experiments gave comparable results.

    Article Snippet: The specific PC-PLC inhibitor D609 ( ) was from Kamiya (Thousand Oaks, CA).

    Techniques: Activity Assay, High Performance Thin Layer Chromatography, Immunostaining